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plenti cas9 gfp plasmid  (Addgene inc)


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    Addgene inc plenti cas9 gfp plasmid
    Plenti Cas9 Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+cas9+gfp+plasmid/pLenti-Cas9-GFP+(Plasmid+%2386145)/pmc13044932-36-27-29
    Average 93 stars, based on 40 article reviews
    plenti cas9 gfp plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Diverse AR Gene Rearrangements Mediate Resistance to Androgen Receptor Inhibitors in Metastatic Prostate Cancer
    Article Snippet: The lentiGuide-Puro plasmid was a gift from Feng Zhang (Addgene plasmid # 52963). .. The pLenti-Cas9-GFP plasmid was a gift from David Sabatini (Addgene plasmid # 86145). ..

    Article Title: Overexpression screen of interferon-stimulated genes identifies RARRES3 as a restrictor of Toxoplasma gondii infection
    Article Snippet: Recombinant DNA reagent , plentiCRISPRv2 (plasmid) , Addgene , 52,961 , . .. Recombinant DNA reagent , pLenti-Cas9-GFP (plasmid) , Addgene , 86,145 , . .. Recombinant DNA reagent , pTRIP.RARRES3 (plasmid) , PMID: 21478870 , pTRIP.CMV.IVSb.RARRES3.ires.TagRFP , .

    Recombinant:

    Article Title: Overexpression screen of interferon-stimulated genes identifies RARRES3 as a restrictor of Toxoplasma gondii infection
    Article Snippet: Recombinant DNA reagent , plentiCRISPRv2 (plasmid) , Addgene , 52,961 , . .. Recombinant DNA reagent , pLenti-Cas9-GFP (plasmid) , Addgene , 86,145 , . .. Recombinant DNA reagent , pTRIP.RARRES3 (plasmid) , PMID: 21478870 , pTRIP.CMV.IVSb.RARRES3.ires.TagRFP , .

    Clone Assay:

    Article Title: Endogenous promoter G-quadruplexes scaffold apurinic/apyrimidinic endonuclease (APE1) to drive gene expression
    Article Snippet: .. Guide RNAs (gRNAs) targeting APE1, C-X-C motif chemokine ligand 1 (CXCL1), and vascular endothelial growth factor A (VEGFA) were designed using Benchling software and cloned into the pLenti-Cas9-GFP plasmid (Addgene #86 145). .. For APE1 knockout, plasmids expressing APE1-targeting gRNAs and Cas9 were transfected into TNBC cells using Lipofectamine 3000 (Invitrogen).



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    Image Search Results


    Parental cell lines RCC-MF, RCC4, and 769-P were each transfected with plasmids of pLenti-Cas9-GFP and were either provided with control sg-RNA or with sg-RNA for VHL . The loss of pVHL expression each VHL KO cell line was confirmed by Western blot.

    Journal: bioRxiv

    Article Title: Rescuing the Function of Missense-Mutated Tumor Suppressor VHL using Stabilizing Small Molecules

    doi: 10.64898/2026.01.02.697373

    Figure Lengend Snippet: Parental cell lines RCC-MF, RCC4, and 769-P were each transfected with plasmids of pLenti-Cas9-GFP and were either provided with control sg-RNA or with sg-RNA for VHL . The loss of pVHL expression each VHL KO cell line was confirmed by Western blot.

    Article Snippet: VHL knockout cells were generated by using Fugene lipofectamine from Promega (# E5911) to transfect cells with pLenti-Cas9-GFP (Addgene, # 83165) and sgRNA for VHL (Sg.VHL_1: 5‘-CGCGGAGGGAATGCCCCGGA-3’, Sg.VHL_2: 5‘-CCTCGGCGCCCAGTTCCTCC-3’, Sg.VHL_3: 5‘-CCTCCCCGCCGTCTTCTTCA-3’).

    Techniques: Transfection, Control, Expressing, Western Blot